Journal: Frontiers in Genetics

Article Title: Gene Expression Profiling during Murine Tooth Development

doi: 10.3389/fgene.2012.00139

Figure Lengend Snippet: Validation of microarray data . The mean ratios (expression at E11.5 up to P7/expression at E11.5) for some of the differentially expressed (DE) genes ( Amelx , Ambn , Enam , Wif1 , Clu , Prnp , Mmp20 , Col1a1 , Tgfb1 , Wint6 , and Serpinb5 ) from the microarray data (black) and real-time RT-PCR (gray). Plotted microarray and real-time RT-PCR data are means derived from at least three biological triplicates at each time point and presented with SD.

Article Snippet: Profile search function of Spotfire software v.9 Decision Site for Microarray analysis software (TIBCO Spotfire, Somerville, MA, USA) was used to select differentially expressed (DE) genes with a similar expression pattern to that of pre-selected genes Ambn , Amelx , and Enam .

Techniques: Biomarker Discovery, Microarray, Expressing, Quantitative RT-PCR, Derivative Assay

Journal: Circulation. Heart failure

Article Title: Differential microRNA-21 and microRNA-221 upregulation in the biventricular failing heart reveals distinct stress responses of right versus left ventricular fibroblasts

doi: 10.1161/CIRCHEARTFAILURE.119.006426

Figure Lengend Snippet: (A)Volcano plot of miRNA expression in RV-HF vs RV-Ctrl. Blue dots, miRNAs that were differentially expressed at P<0.10. Labeled dots, miRNAs that were differentially expressed at a minimum 2-fold change in either direction (n=3 per group). (B)Heat maps, Venn diagram, and summary bar graph of differentially expressed miRNAs. Orange font, differentially expressed in RV-HF vs RV-Ctrl and in LV-HF vs LV-Ctrl but not statistically significantly different in RV-HF vs LV-HF. Blue font, differentially expressed in RV-HF vs RV-Ctrl and in RV-HF vs LV-HF, but not statistically significantly different in LV-HF vs LV-Ctrl. Purple font, differentially expressed across all three comparisons: LV-HF vs LV-Ctrl, RV-HF vs RV-Ctrl, and RV-HF vs LV-HF. *P<0.05 vs respective LV-HF/LV-Ctrl. (C)Quantitative RT-PCR analysis of miR-21 and miR-221 in ventricular tissue, n= 6 per group. *P<0.01 vs respective Ctrl; #P<0.01 vs LV HF. (D)Cyclic overstretch and/or aldosterone induced a marked increase in miR-21 (*P<0.01 vs unstimulated) and (E)miR-221 (*P<0.05 vs unstimulated) only in RV fibroblasts. (F)Inhibition of miR-21/−221 attenuated proliferation in RV but not LV fibroblasts. n= 4 per experimental condition. *P<0.05 vs respective LV, #P<0.05 vs RV without antimir, analyzed by ANOVA on Ranks.

Article Snippet: miRNA microarray data was analyzed for differential miRNA expression between pre-specified groups (RV-HF vs. RV-Ctrl, LV-HF vs. LV-Ctrl, and RV-HF vs. LV-HF) by two-tailed Student’s t-test using GraphPad Software (Prism 7.0).

Techniques: Expressing, Labeling, Quantitative RT-PCR, Inhibition